李长有 林国宏 林国宏 基础医学院病理学教研室；李长有 中国医科大学第一临床学院骨科，沈阳 110001 中国医科大学学报 1999 0 28 5


    关键词：脊髓；缺血；免疫组织化学 期刊 zgykdxxb 0 临床医学 fur -->


    

摘要 目的： 应用免疫组织化学方法观察实验性兔腰髓缺血40 min、再灌流4 h的脊髓运动神经元胞体和轴突的病理学改变。方法：采用神经元特异性烯醇酶(NSE)抗体和神经中丝(NF)抗体分别特异标记神经元胞体和轴突，结果进行图象分析。结果：缺血40 min，前角运动神经元胞体和轴突内NSE和NF反应异常地增强，胞体内NSE和NF分布紊乱聚集。再灌流4 h，前角运动神经元胞体和轴突内反应阳性，胞体内分布散乱、稀疏、崩溃和溶解，轴突肿胀、扩大、消失。图象分析前角运动神经元胞体内NSE和NF的面积、灰度和前索内轴突的数量，其结果具有明显的统计学意义。结论：免疫组织化学方法能更清楚地显示脊髓运动神经元胞体和轴突的病理学改变。

The Immunohistochemical Evaluation in Experimental Ischemic Spinal Cord Injury

Li Changyou, Lin Guohong

Department of Orthopaedic Surgery,The First Clinical College,China Medical University,Shenyang,110001

ABSTRACT Objective: The paper was to evaluate possible significances of immunohistological method in assessing experimental ischemic damage on the spinal cord.Methods: Neuron specific endolase(NSE) and neurofilament(NF) antibody were used to specifically label cell bodies and axons of the neuron in the spinal cord,and results were analysed by the methods of the image pattern analyis.Results: The excessive misaccumulation of NSE and NF were observed in the cell bodies and axons of motorneurons in ventral horn after 40 min of ischemia, and the distributions of NSE and NF in the cell bodies were accumulative. The NSE and NF immunohistochemical reaction could be shown in the cell bodies and axons of motorneuorns after 4 h of reperfusion,and the distributions were divergent,rare and disintegrative,the axons were swollen and vanish. The areas,grey levels of NSE and NF in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference.Conclusion: The pathological changes of cell bodies and axons of motorneurons were demonstrated clearly by immunohistochemical reaction.

     KEY WORDS spinal cord; ischemia; immunohistochemistry

免疫组织化学方法在实验性脊髓损伤评价方面的报道较少 ......